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Liquid Chromatographic behavior of Ni(Ⅱ) in Isonitrosoethylacetoacetate Imine(IEAA-NR), Ni(IEAA-NH)(IEAA-NR)(R = H, CH3, C2H5, n-C3H7, n-C4H9, C6H5-CH2) chelates were investigated by reversed-phase HPLC on Micropak MCH-5 column using methanol/water as mobile phase. The optimum conditions for the separation of Ni(IEAA-NH)(IEAA-NR) chelates were examined with respect to the effect of the flow rate, sample solvent, mobile phase strength and column temperature. It was found that metal chelates were properly eluted in an acceptable range of capacity factor value(0≤logk'≤1). The dependence of the logarithm of capacity factor (k') on the volume fraction of water in the binary mobile phase as well as on the liquid-liquid extraction distribution ratio (Dc) in methanol-water/n-alkane extraction system showed the good linearties, and the dependence of the logarithm of capacity factor (k') on the column temperature and on the enthalpy exhibited the good linearties, and the compensation temperature (β) from the slope was 773.47˚K. It was suggested that the retention of metal chelates was largely affected by the hydrophobic effect.
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